A549-EGFP cell line

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A549-EGFP Order

A549-EGFP cell line
Catalog#
YC-C016-EGFP-P
Size
1*10^6
Transfection Culture Medium
Transfection Culture Medium(A549)
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Datasheet
A549-EGFP cell line
Order

Detailed Product Information

Product category
EGFP Stable Cell Lines
cell name
A549-EGFP
Cell Morphology
Epithelial-like, adherent
Passage ratio
1:3~1:4
Species
Human
Reporter gene
EGFP
Resistance gene
puro
Construction method
Lentivirus
Mycoplasma test
negative
Culture method
90% F12K+10% FBS
Accession
CVCL_XB31(Cellosaurus)
ExPASy Cellosaurus Database
Is a valuable online resource that compiles detailed information on over 100,000 cell lines from reputable cell banks such as ATCC, ECACC, and DSMZ. The database provides information including descriptions, origins, and characteristics of the cell lines, with broad reference value and scientific reliability. Additionally, the database provides information on cell line authentication, STR profiles, and potential contamination risks, helping researchers ensure the trustworthiness of the cell lines used in their experiments.
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:A549		 STR Info (Cell bank)
Cell Line:A-549
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11111111
D13S317 11111111
D7S820 811811
D16S539 11121112
VWA 14141414
TH01 89.389.3
AMEL XYXY
TPOX 811811
CSF1PO 10121012
D12S391 1818
FGA 2323
D2S1338 2424
D21S11 2929
D18S51 1417
D8S1179 1314
D3S1358 1616
D6S1043 1113
PENTAE 711
D19S433 1313
PENTAD 99
D1S1656 1718.3
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.
EGFP validation
A549-EGFP cell line EGFP validation

Instruction

Use EGFP gene expressing lentivirus constructed by Ubigene to infect the A549 cell line， screen the cells based on the puromycin resistance marker and the optimal antibiotic screening concentration explored in advance, resulting in a A549-EGFP cell line that can maintain long-term, stable and high expression.
A549-EGFP cell line
A549-EGFP
Catalog#
YC-C016-EGFP-P
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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